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    Subjects/Microbiology/Leishmania — Kala-azar
    Leishmania — Kala-azar
    medium
    bug Microbiology

    A 35-year-old man from Bihar presents with fever, hepatosplenomegaly, and pancytopenia for 3 months. Bone marrow examination reveals Leishman–Donovan bodies. Which feature best distinguishes visceral leishmaniasis (kala-azar) from cutaneous leishmaniasis?

    A. Amastigote stage visible on Giemsa staining
    B. Ulcerating skin lesion at the site of sandfly bite
    C. Positive leishmanin skin test (Montenegro test)
    D. Presence of Leishman–Donovan bodies in macrophages of reticuloendothelial organs

    Explanation

    ## Distinguishing Features of Visceral vs Cutaneous Leishmaniasis **Key Point:** The hallmark of kala-azar is dissemination of *Leishmania donovani* to the reticuloendothelial system (liver, spleen, bone marrow, lymph nodes), where amastigotes multiply within macrophages as Leishman–Donovan (LD) bodies. This systemic involvement is pathognomonic for visceral leishmaniasis. ### Comparative Table: Visceral vs Cutaneous Leishmaniasis | Feature | Visceral (Kala-azar) | Cutaneous | | --- | --- | --- | | **Parasite species** | *L. donovani* | *L. tropica*, *L. major*, *L. mexicana* | | **Site of LD bodies** | Macrophages of liver, spleen, bone marrow, lymph nodes | Dermis and epidermis at lesion site | | **Clinical presentation** | Fever, hepatosplenomegaly, pancytopenia, wasting | Ulcerating nodule/plaque at bite site | | **Leishmanin test** | Negative (anergy) | Positive (cell-mediated immunity) | | **Organomegaly** | Massive (up to 10 kg splenomegaly) | Absent | | **Diagnostic site** | Bone marrow, splenic aspirate | Lesion margin biopsy | **High-Yield:** The *negative leishmanin test in kala-azar* reflects immune suppression by the parasite, whereas cutaneous leishmaniasis shows a positive test due to intact cell-mediated immunity. **Clinical Pearl:** LD bodies are found in abundance in visceral leishmaniasis (especially splenic aspirate — 95% sensitivity) but are sparse or absent in cutaneous disease, making this the single best discriminator. ### Why This Matters - Visceral leishmaniasis is a systemic infection with high mortality (>90%) if untreated; cutaneous disease is localized and self-limiting in most cases. - Bone marrow or splenic aspirate showing LD bodies confirms kala-azar diagnosis; their absence in cutaneous lesions is expected. [cite:Park 26e Ch 8]

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