Answer- C. Apple green birefringenceAmyloid has an affinity for Congo red stain; therefore this method is used for confirmation of amyloid of all types. If the stained section is viewed in polarised light, the amyloid characteristically shows apple-green birefringence due to cross- b-pleated sheet configuration of amyloid fibrils.The stain can also be used to distinguish between AL and AA amyloid (primary and secondary amyloid respectively).
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