Entamoeba histolytica

A 38-year-old man from rural Bihar presents with a 3-week history of bloody diarrhea, abdominal pain, and fever. Stool microscopy shows trophozoites with ingested RBCs. What is the most appropriate next investigation to confirm the diagnosis and assess for extraintestinal involvement?

A 42-year-old woman from Kolkata presents with right upper quadrant pain, fever (38.5°C), and hepatomegaly. Ultrasound shows a 6 cm hypoechoic lesion in the right lobe of the liver with internal echoes. Stool microscopy is negative for parasites. Which investigation is most specific for confirming amoebic liver abscess?

A 32-year-old man from rural Bihar presents with a 3-week history of bloody diarrhoea, abdominal pain, and tenesmus. He reports consuming contaminated water from a hand pump. On examination, he is afebrile with mild abdominal tenderness. Stool microscopy reveals motile trophozoites with ingested RBCs. Blood culture is negative. What is the most likely diagnosis?

The microscopic image above shows a trophozoite with characteristic features. This finding is most consistent with:

A 38-year-old man from rural Bihar presents with a 3-week history of bloody diarrhoea, tenesmus, and lower abdominal pain. Stool microscopy shows motile trophozoites with ingested RBCs. What is the investigation of choice to confirm the diagnosis of invasive amoebiasis and assess for extraintestinal complications?