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    Subjects/Microbiology/Culture Media and Growth
    Culture Media and Growth
    medium
    bug Microbiology

    A 28-year-old woman from rural Maharashtra presents to a TB clinic with a 3-month history of persistent cough, fever, and weight loss. Sputum smear microscopy is negative for acid-fast bacilli (AFB) on direct examination. The laboratory technician suspects *Mycobacterium tuberculosis* but wants to culture the specimen to confirm diagnosis and perform drug susceptibility testing. Which of the following culture media would be the MOST appropriate choice for primary isolation of *M. tuberculosis* from this sputum sample?

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