A 5-year-old boy from northern India presents with bowing of legs, frontal bossing, rachitic rosary, and delayed tooth eruption. Serum calcium is 7.8 mg/dL, phosphate 3.2 mg/dL, alkaline phosphatase 280 IU/L, and PTH 210 pg/mL. Which investigation is most specific for confirming nutritional rickets and assessing severity?

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