Oxidative Phosphorylation Uncouplers

A 28-year-old male with a history of chronic DNP (2,4-dinitrophenol) abuse for weight loss presents with progressive weakness, paresthesias in both feet, and a core temperature of 39.2°C despite ambient temperature of 22°C. He reports taking DNP capsules irregularly over the past 6 months. Neurological examination reveals distal sensory loss and diminished ankle reflexes. What is the most appropriate immediate next step in management?

A 28-year-old man with a history of accidental exposure to 2,4-dinitrophenol (DNP) 6 weeks ago presents with persistent unexplained fever (37.8–38.5°C), profuse diaphoresis, and a resting metabolic rate 40% above predicted. Standard investigations (CBC, CMP, blood cultures, thyroid function) are all normal. The clinical team suspects ongoing mitochondrial uncoupling. Which investigation would best assess the current degree of mitochondrial uncoupling and guide therapeutic intervention?

A 28-year-old woman with a history of severe obesity is referred to the endocrinology clinic. Genetic testing reveals a homozygous loss-of-function mutation in the UCP1 gene. Which of the following is the most common metabolic consequence of this mutation?

Which of the following is the most common physiological uncoupler of oxidative phosphorylation in mammalian mitochondria?

Thermogenin (UCP1) in brown adipose tissue acts as an uncoupler by which of the following mechanisms?