PCR and Blotting Techniques

All of the following statements regarding the polymerase chain reaction (PCR) are correct EXCEPT:

Which of the following is the primary function of Taq polymerase in PCR?

In a Southern blot, what is the role of the restriction enzyme?

A 32-year-old woman with a family history of cystic fibrosis (CF) is undergoing genetic screening. Initial mutation analysis by PCR detected the ΔF508 mutation in one allele. To identify the second mutation and confirm compound heterozygosity, which investigation is most appropriate?

All of the following are correct regarding Southern blotting EXCEPT: